Part:BBa_K4501022

pJUMP lentiviral transfer plasmid

Disclaimer. This is a plasmid backbone and therefore the compatibility labels are not applicable. This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The modules (BBa_K4501005 and BBa_K4501006) are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.

Figure 1. Map sequence of the pJUMP-based lentiviral transfer plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 5061
  Illegal suffix found at 5105
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5061
  Illegal SpeI site found at 5106
  Illegal PstI site found at 5120
  Illegal NotI site found at 5067
  Illegal NotI site found at 5113
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5061
  Illegal BglII site found at 33
  Illegal BglII site found at 74
  Illegal BglII site found at 3682
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5061
  Illegal suffix found at 5106
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5061
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5076
  Illegal SpeI site found at 5106
  Illegal PstI site found at 5120
  Illegal NgoMIV site found at 1895
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 2693
  Illegal SapI.rc site found at 4194